RSS Feed for molecular typingCategory: molecular typing

First assigned reading of Spring ‘08: explaining extremophile biogeography »

Is everything everywhere?  How much does the environment select?  Are there barriers to dispersal for microbes? We’re still working on these questions, so to help us focus we will dig into our first reading(s) (one mandatory, one optional).  They are:
1. http://www.ncbi.nlm.nih.gov/pubmed/12881573?dopt=Abstract
and
2. (optional) http://www.ncbi.nlm.nih.gov/pubmed/16093568?dopt=Abstract
Both are posted in full under the Assignments page.
For this week, please read [...]

First class of ‘08: is everything everywhere? »

Hi gang,
I’ve prepared an overview of several recent studies that attempt to answer this question about microbial communities. The slides are posted on the assignments blog, at
http://micropopbio.org/cooper/2008/01/24/micropopbio-lecture-1/
For your reference, the articles that I refer to in this lecture can be found at:
http://aem.asm.org/cgi/content/abstract/66/12/5448
http://www.pnas.org/cgi/content/abstract/104/8/2761
http://www.nature.com/nature/journal/v432/n7018/abs/nature03073.html

Lecture notes for 9.13.07 »

Today we covered:
The Cho and Tiedje paper, “Biogeography and degree of endemicity of fluorescent Pseudomonas strains in soil”, AEM. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=11097926
This article relied heavily on two methods: BOX-PCR, a molecular typing method, and UPGMA, a method of estimating similarity and indirectly, phylogenetic distance. Good descriptions for each of these can be found at:
BOX-PCR (in the context [...]