Samantha

Hey guys I think you may have gone over this paper in class while I was sick but Vaughn told me to summarize it so here i go:

-They took GJVI which was a clone of the standard lab strain DK1622 = The ANCESTOR

-They evolged 12 lineages for 1000 generations in nutrient-rich medium. There was no positive selection on any social traits (social motility or social development) only on competitiveness in asocial growth conditions
~Replicate lineages improved maximum growth rates by about 37%
~There was a partial to complete loss of the capasity for social motility and development

-After 1000 generations several evolved clones that were able to cheat on the ancestor in a mixed population were isolated
~There was defective sporulation in clonal culture
~But they are more efficient than the ancestor in a mixed population (when rare)
1. One of the cheating clones is the OC (obligate cheater)
2. OC is completely defective at social development during starvation and needs other socially proficient to sporulate
3. OC is marked with Kanamycin resistance

-OC w/ Kan resistance was mixed with a marked Rif resistanct variant of GJVI

-They did 6 sequential cycles of alternating starvation and growth
~After 4 cycles, OC re-evolved the ability of multicellular development which resulted into the PX genotype

-They sequenced PX in 2 ways
~Pico-Titer-Plate
~Capillary-based

-Compared against DK1622
~27 real discrepencies found

-Then they compared PX to three other strains:
~GJVI (identical clone of DK1622)
~GVB207.3 (unmarked immediate parent of OC isolated from 1000 generations evolved from GJVI)
~OC (evolutionary ancestor of PX)
1. 26 of the 27 discrepencies we also present in GVB207.3 and OC
2. 1 discrepency was unique to PX (phenotypic transition from OC—> PX